Comparing the genomes of Helicobacter pylori clinical strain UM032 and Mice-adapted derivatives

  • Yalda Khosravi1,

    Affiliated with

    • Vellaya Rehvathy1,

      Affiliated with

      • Wei Yee Wee2,

        Affiliated with

        • Susana Wang3,

          Affiliated with

          • Primo Baybayan3,

            Affiliated with

            • Siddarth Singh4,

              Affiliated with

              • Meredith Ashby3,

                Affiliated with

                • Junxian Ong5,

                  Affiliated with

                  • Arlaine Anne Amoyo5,

                    Affiliated with

                    • Shih Wee Seow5,

                      Affiliated with

                      • Siew Woh Choo2,

                        Affiliated with

                        • Tim Perkins6,

                          Affiliated with

                          • Eng Guan Chua7,

                            Affiliated with

                            • Alfred Tay7,

                              Affiliated with

                              • Barry James Marshall7,

                                Affiliated with

                                • Mun Fai Loke1,

                                  Affiliated with

                                  • Khean Lee Goh8,

                                    Affiliated with

                                    • Sven Pettersson5, 9, 10 and

                                      Affiliated with

                                      • Jamuna Vadivelu1Email author

                                        Affiliated with

                                        Gut Pathogens20135:25

                                        DOI: 10.1186/1757-4749-5-25

                                        Received: 2 July 2013

                                        Accepted: 16 August 2013

                                        Published: 19 August 2013



                                        Helicobacter pylori is a Gram-negative bacterium that persistently infects the human stomach inducing chronic inflammation. The exact mechanisms of pathogenesis are still not completely understood. Although not a natural host for H. pylori, mouse infection models play an important role in establishing the immunology and pathogenicity of H. pylori. In this study, for the first time, the genome sequences of clinical H. pylori strain UM032 and mice-adapted derivatives, 298 and 299, were sequenced using the PacBio Single Molecule, Real-Time (SMRT) technology.


                                        Here, we described the single contig which was achieved for UM032 (1,599,441 bp), 298 (1,604,216 bp) and 299 (1,601,149 bp). Preliminary analysis suggested that methylation of H. pylori genome through its restriction modification system may be determinative of its host specificity and adaptation.


                                        Availability of these genomic sequences will aid in enhancing our current level of understanding the host specificity of H. pylori.


                                        Helicobacter pylori PacBio Single Molecule Real-Time (SMRT) technology Clinical H. pylori Mice-adapted


                                        Helicobacter pylori persistently colonizes the human stomach to cause chronic gastritis, peptic ulcer disease, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma [1]. The mechanisms involved in the pathogenesis of H. pylori infections are still not fully established [2]. Thus, experimental animal models that mimic human diseases are essential to provide information on etiopathogeny, immunity and therapy, as well as to improve our understanding on ways H. pylori can induce a diverse range of gastric pathologies [3, 4]. Among various animal models available, mouse remains the most readily used animal model for studying H. pylori-induced diseases and have played important roles in the elucidation of factors required for colonization, distribution and persistence of infection [2].


                                        Mice adaptation study

                                        Adopting a similar strategy as in previous studies [2, 5], a pool consisting of twelve clinical strains of H. pylori isolated from patients presenting for gastroscopy at the University of Malaya Medical Centre (UMMC) was inoculated intragastrically into five 4–6 weeks old male C57BL/6 mice. Multiple colonies of H. pylori were successfully recovered from the gastric tissue sample of a mouse (1/5) following necropsy two weeks post-infection. Random amplification of polymorphic DNA (RAPD) fingerprinting was used to trace back the mice-adapted isolates to its parental clinical strain, UM032. H. pylori UM032 was isolated from a patient presenting with peptic ulcer disease. Mice-adapted isolates from the first mouse passage were designated as 298 and were used for the second round of mouse passage to access the stability and infectivity of this mice-adapted strain. All three mice inoculated with the mice-adapted 298 strain were successfully infected and H. pylori isolated from the second passage were designed as 299. The animal study was performed with the approval of the SingHealth Institutional Research Committees (SHS IBC) and the Ethical Committee for Animal Research (Form No. SHS-IBC-201, January 2010).

                                        Genome sequencing

                                        In this study, H. pylori DNA was isolated using the RTP Bacteria DNA Mini Kit (Invitek GmbH, Berlin, Germany). The extracted DNA samples were sequenced using Pacific Biosciences RS sequencing technology (Pacific Biosciences, Menlo Park, CA), yielding >20× average genome coverage. Each sample was prepared as a 10-kb insert library using C2 chemistry and sequenced on 8 Single-Molecule Real-Time (SMRT) cells.

                                        Assembly and annotation

                                        De novo assembly of the read sequences was created using the continuous long reads (CLR) following the Hierarchical Genome Assembly Process (HGAP) workflow (http://​pacbiodevnet.​com/​) as available in SMRT Analysis v2.0. The genomes were annotated with the NCBI (National Center for Biotechnology Information) Prokaryotic Genomes Automatic Annotation Pipeline and NMPDR (National Microbial Data Resource) Rapid Annotation using Subsystem Technology (RAST) [6]. The SEED-Viewer was used to visualize the genome annotation and comparison generated by RAST [7].

                                        Submission of genome sequence

                                        The genome sequence of the Helicobacter pylori strains UM032, 298 and 299 are available in DDBJ/EMBL/GenBank under Accession numbers CP005490, CP006610 and CP005491 respectively.

                                        Quality assurance

                                        The genomic DNA was isolated from pure bacterial isolate (positive for urease, catalase and oxidase tests) and was further confirmed with 16SrRNA sequencing and genotyping of bacterial virulence factors. Bioinformatic assessment of potential contamination of the genomic library by allochthonous microorganisms was done using PGAAP and RAST annotation systems.

                                        Initial findings

                                        Genome characteristics

                                        Based on the assembled genomes with HGAP using PacBio long reads from a single library preparation, single contigs were achieved for UM032 (1,599,441 bp), 298 (1,604,216 bp) and 299 (1,601,149 bp). The GC content for all three assembled genomes was 38.8%. Additional information is included in the sequencing reports: UM032 (Additional file 1), 298 (Additional file 2) and 299 (Additional file 3). Figure 1 describes the subsystem distribution of the parental clinical strain, UM032. Figure 2 shows the sequence homology between 298, 299 and Shi470 with reference to UM032. H. pylori Shi470 was predicted to be among those closest to UM032 with score of 470. Neither gene lost nor gain was found in the mice-adapted derivatives (298 and 299) when compared to the parental strain (UM032). Interestingly, total base modifications detected through the PacBio RS sequencing platform as described under Table 1was reducing with passaging in mice. In addition, a 348 b.p. gene encoding for a putative type IIS restriction modification (R-M) enzyme in H. pylori UM032 was found in to truncated in 298 and 299 (Figure 3). Thus, methylation may be a mean of host adaptation by H. pylori and may have an important role in determining host specificity.
                                        Figure 1

                                        Subsystem distribution statistic of Helicobacter pylori strain UM032 based on genome annotation performed according to RAST server.

                                        Figure 2

                                        Genome sequence comparison of Helicobacter pylori 298 (outer) 299 (middle) and Shi470 (inner) when aligned with reference genome, UM032, using RAST program. Intensity of color indicates degree of protein identity (legend).

                                        Table 1

                                        Type of base modifications and associated motifs detected


                                        Modification type

                                          # of motifs detected





                                        GA NTC





                                        CCA TC





                                        GA GG










                                        GA TC





                                        C CGG










                                        CYA NNNNNNNTRG





                                        ATTAA T





                                        AC NGT





                                        CA TG





                                        GAAA G















                                        Figure 3

                                        Pair-wise alignment of putative type IIS restriction modification enzyme. Deletion of single guanine nucleotide at position 214 resulted in downstream frame-shift mutation and prematured termination of the RM enzymes encoded by 298 and 299.

                                        The availability of complete sequences of mice-adapted strains and their parental clinical isolate will provide important information that contributes towards our understanding of the host specific and adaptation of H. pylori. In addition, it will help in extrapolate results obtained using mice model to the natural human host of H. pylori. H. pylori 298 strain will be used for H. pylori colonizing studies in mice.

                                        Putative gene clusters responsible for survival and virulence of H. pylori

                                        H. pylori possess genes for cytosolic urease biosynthesis, which is governed by a seven-gene cluster, are essential for its survival in the acidic gastric environment [8]. H. pylori vacuolating cytotoxin A (Vac A) is an important virulence factor of the bacterium [9]. Using the SEED database, genetic relatedness of the urease gene cluster and vac A for the clinical strain (UM032) and its mice-adapted counterparts (298 and 299) in comparison to other known H. pylori strains are shown in Figures 4 and 5.
                                        Figure 4

                                        Genetic relatedness of urease gene cluster with closely related bacteria. 1: urease beta subunit/urease gama subunit, 2: cell division protein Ftsk, 3: outer membrane protein, 4: lipoprotein signal peptidase, 5: urease alpha subunit, 6: phosphoglucosamine mutase, 7: urea channel ure I, 8: SSU ribosomal protein S20P, 9: urease accessory protein ure E, 10: peptide chain release factor I, 11: urease accessory protein ure F, 12: urease accessory protein ure G, 13: urease accessory protein ure D, 14: dentin sialophosphoprotein preproprotein.

                                        Figure 5

                                        Genetic relatedness of vac A cluster with closely related bacteria. 1: vacuolating cytotoxin, 2: hypothetical protein, 3: haemin uptake system ATP-binding protein, 4: cysteinyl-Trna-SYNTHETASE, 5: IRON III, 6: dehydrogenases with different specificities, 7: proposted peptidoglycan lipid, 8: hypothetical protein, 9: hypothetical protein, 10: DNA damage inducible protein J, 11: holliday junction DNA helicase RUUA, 12: putative outer membrane protein, 13: hypothetical protein.

                                        Future directions

                                        To our knowledge, this is the first genome sequence of H. pylori isolated from human and mouse using PacBio SMRT Technology. Comparative genomic and more-detailed methylomic analysis of these data is in process and will be included in future publications. Mice-adapted H. pylori described here will be used in future H. pylori infection studies in mice.

                                        Availability of supporting data

                                        The data sets supporting the results of this article are included within the additional files.



                                        Funding was provided by the University of Malaya-Ministry of Higher Education (UM-MOHE) High Impact Research (HIR) grant (reference UM.C/625/1/HIR/MOHE/CHAN-02; “Molecular Genetics”). SW, PB, SS and MA are full-time employees at Pacific Biosciences, the commercial company for the SMRT sequencing technologies. We thank the NCBI (National Center for Biotechnology Information) rapid annotation pipeline team for providing the genome annotation services.

                                        Authors’ Affiliations

                                        Department of Medical Microbiology, University of Malaya
                                        Dental Research and Training Unit, Faculty of Dentistry, University of Malaya
                                        Pacific Biosciences
                                        PacBio Singapore
                                        National Cancer Centre
                                        School of Pathology and Laboratory Medicine, Faculty of Medicine, Dentistry and Health Sciences, University of Western Australia
                                        The Marshall Centre for Infectious Diseases Research and Training, University of Western Australia
                                        Department of Medicine, University of Malaya
                                        Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet
                                        School of Biological Sciences, Nanyang Technological University


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