Figure 2From: Exposure to bile influences biofilm formation by Listeria monocytogenesBioluminescence imaging of biofilm. L. monocytogenes EGDe lux cells that were grown in BHI (- bile) or BHI containing 0.3% oxgall (+ bile) were washed in Ringer's solution and inoculated at equal cell numbers into fresh BHI broth. 3 ml was added to individual wells of a 6-well plate. After (A) 4 hours and (B) 24 hours the contents of wells were removed and loosely attached bacteria were removed by washing with distilled water. 1 ml of fresh BHI broth was added and luminescence was measured in a Xenogen IVIS100. The color bar indicates bioluminescence signal intensity (in photons s-1 cm-2). Results shown are those obtained for three biological repeats in one particular experiment and are representative of three independent experiments.Back to article page