Figure 3
From: Campylobacter jejuni induces transcellular translocation of commensal bacteria via lipid rafts
C. jejuni promotes association of E. coli with lipid rafts. (A) Lipid rafts were isolated by sucrose gradient fractionation and fluorescent E. coli in each fraction was measured (values are shown as relative fluorescence units (RFU)). Arrows indicate fractions containing lipid raft-associated E. coli. *P < 0.05 compared to control and cholesterol-depleted monolayers. Data are means ± SEM (n = 3). (B) Fractions were analyzed by western blot analysis for the lipid raft marker, caveolin-1. A representative image from three experiments is shown.