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Table 1 MIC measurements, regression parametersa and statistical analysis of the dose-response curves illustrated in Figure 1

From: Penicillin-binding Proteins (PBP) and Lmo0441 (a PBP-like protein) play a role in Beta-lactam sensitivity of Listeria monocytogenes

  AMP MEM CFX
  MIC (mg/L) Emaxb (CI)c Δlog from original inoculum R2 MIC (mg/L) Emaxb (CI)c Δlog from original inoculum R2 MIC (mg/L) maxb (CI)c log from original inoculum R2
EGDe WT 0.125 -1.53 (-1.7 to -1.3) 0.97 0.0625 -1.85 (-2.2 to -1.5) 0.95 8 -3.65 (-4.2 to -3.1) 0.97
ΔPBP4 0.125 -2.51 (-2.7 to -2.3)d 0.98 0.0625 -2.92 (-3.3 to -2.5)d 0.98 2 -2.29 (-2.6 to -1.9)d 0.93
ΔLmo0441 0.125 -1.74 (-1.9 to -1.5) 0.97 0.0625 -2.02 (-2.5 to -1.6) 0.97 2 -1.92 (-2.2 to -1.7)d 0.95
  1. Ampicillin (AMP), meropenem (MEM) and cefuroxime (CFX) MICs measurements for the parental strain EGDe and ΔPBP4 and ΔLmo0441 mutant strains. MICs of AMP and MEM, with a respective value of 0.125 and 0.0625 mg/L, did not differ between wild type and mutant strains. However, there is a 4-fold increase in MIC of CFX for both mutant strains, which passes from 8 mg/L to 2 mg/L, a finding which is in agreement with previous observations [9].
  2. a Regression parameters are based on the Hill equation (using a variable slope)
  3. b Concentration causing maximal activities (Emax values), as obtained from the Hill equation. Values are expressed as a multiple of the respective MIC for a given strain.
  4. c CI - Confidence Intervals (95%)
  5. d Significantly different (p < 0.05) from the wild type strain as calculated by F-test