Stimulant | IFN-γ SFUs/10e6 cells | p-value |
---|
| AroC+GFP Group | AroC+pGEM Group | |
---|
Media | 6 ± 2 | 7 ± 5 | 0.84 |
GFP CD8 peptide | 232 ± 15 | 6 ± 2 | 0.0001 |
GFP protein | 4 ± 0 | 4 ± 2 | 1.00 |
Salmonella LPS | 244 ± 39 | 375 ± 62 | 0.037 |
Vaccine group
|
Media
|
GFP peptide
|
p-value
|
AroC+GFP | 6 ± 2 | 233 ± 15 | 0.0001 |
AroC+pGEM | 7 ± 5 | 6 ± 2 | 0.70 |
- Groups of mice were vaccinated three times (Days 0, 28 and 56) with live recombinant Salmonella vaccine expressing GFP (AroC+GFP) or a negative Salmonella control vaccine not expressing any antigen (AroC+pGEM). On Day 84 (28 days after the last inoculation), splenocytes from the sacrificed mice were incubated with media only (negative assay control), or stimulated with GFP CD8+ T cell peptide (HYLSTQSAL), full-length GFP or Salmonella LPS in an IFN-γ ELISPOT assay. The mean number of spots ± SD in triplicate wells was calculated and expressed as IFN-γ SFUs/10e6 cells. Difference in response between or within vaccines was determined. Responses differ significantly if the p-values are less than 0.05 and do not differ significantly if the p-values are greater than 0.05.