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Table 1 The magnitude of GFP-specific CD8+ T cell responses as measured by IFN-γ ELISPOT assay

From: An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice

Stimulant IFN-γ SFUs/10e6 cells p-value
  AroC+GFP Group AroC+pGEM Group  
Media 6 ± 2 7 ± 5 0.84
GFP CD8 peptide 232 ± 15 6 ± 2 0.0001
GFP protein 4 ± 0 4 ± 2 1.00
Salmonella LPS 244 ± 39 375 ± 62 0.037
Vaccine group Media GFP peptide p-value
AroC+GFP 6 ± 2 233 ± 15 0.0001
AroC+pGEM 7 ± 5 6 ± 2 0.70
  1. Groups of mice were vaccinated three times (Days 0, 28 and 56) with live recombinant Salmonella vaccine expressing GFP (AroC+GFP) or a negative Salmonella control vaccine not expressing any antigen (AroC+pGEM). On Day 84 (28 days after the last inoculation), splenocytes from the sacrificed mice were incubated with media only (negative assay control), or stimulated with GFP CD8+ T cell peptide (HYLSTQSAL), full-length GFP or Salmonella LPS in an IFN-γ ELISPOT assay. The mean number of spots ± SD in triplicate wells was calculated and expressed as IFN-γ SFUs/10e6 cells. Difference in response between or within vaccines was determined. Responses differ significantly if the p-values are less than 0.05 and do not differ significantly if the p-values are greater than 0.05.