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Table 4 Quantitative polymerase chain reaction (qPCR) primes used in this study to enumerate specific bacterial species.

From: Luminal and mucosal-associated intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome

Bacterial species

 

Primer Sequence 5'-3'

Annealing temperature (°C)

PCR Product size (bp)

Bacteroides spp.a

F-

R-

ATAGCCTTTCGAAAGRAAGAT

CCAGTATCAACTGCAATTTTA

50

495

Clostridium spp.b

F-

R-

CGGTACCTGACTAAGAAGC

AGTTTYATTCTTGCGAACG

50

429

Escherichia coli c

F-

R-

GTTAATACCTTTGCTCATTGA

ACCAGGGTATCTAATCCTGTT

52

340

Bifidobacterium spp.d

F-

R-

GGGTGGTAATGCCGGATG

TAAGCGATGGACTTTCACACC

55

442

Lactobacillus spp.e

F-

R-

AGCAGTAGGGAATCTTCCA

CACCGCTACACATGGAG

50

341

  1. aprimers from Matsuki et al.,[26]
  2. bprimers from Rinttila et al.,[27]
  3. cprimers from Malinen et al.,[28]
  4. dprimers from Bartoch et al.,[29]
  5. eprimers from Maeda et al.,[30]
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Gut Pathogens

ISSN: 1757-4749