Figure 2
From: Bacteriophage-encoded shiga toxin gene in atypical bacterial host
Agarose gel picture of stx -specific PCR on canine fecal DNA and canine fecal isolates. A) Initial stx-specific PCR screening of DNA extracted from canine fecal samples, B) stx-specific PCR on bacterial DNA extracted from canine fecal samples (n = 3, 10 Ī¼l and 20 Ī¼l loaded), and C) stx-specific PCR showing two of the five positive results from the cultured bacterial isolates. White arrows indicate the stx-positive isolates. White triangle is stx standard curve from 10E5 copies ml-1 to one copy ml-1, neg = negative control, DB #20 = Dog Beach fecal sample #20, 500 bp = 500 base pair DNA ladder. Negative controls consistently had no detectable PCR products.