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Figure 2 | Gut Pathogens

Figure 2

From: Bacteriophage-encoded shiga toxin gene in atypical bacterial host

Figure 2

Agarose gel picture of stx -specific PCR on canine fecal DNA and canine fecal isolates. A) Initial stx-specific PCR screening of DNA extracted from canine fecal samples, B) stx-specific PCR on bacterial DNA extracted from canine fecal samples (n = 3, 10 μl and 20 μl loaded), and C) stx-specific PCR showing two of the five positive results from the cultured bacterial isolates. White arrows indicate the stx-positive isolates. White triangle is stx standard curve from 10E5 copies ml-1 to one copy ml-1, neg = negative control, DB #20 = Dog Beach fecal sample #20, 500 bp = 500 base pair DNA ladder. Negative controls consistently had no detectable PCR products.

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