Figure 3
From: HtrA chaperone activity contributes to host cell binding in Campylobacter jejuni
Effect of mutations in the htrA gene of C. jejuni on secretion of Cia proteins. Secretion of Cia proteins were induced by growing C. jejuni NCTC11168 (wt), LB1281(ΔhtrA) and KB1025 (htrA S197A) in the presence of sodium deoxycholate followed by radioactive labelling in RPMI medium containing FBS. Labeled proteins in the bacterial lysates and in the supernatants were separated by SDS-PAGE and detected by autoradiography. The positions of molecular weight standards are indicated on the right (in kilodaltons).