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Figure 2 | Gut Pathogens

Figure 2

From: Helicobacter pylori phagosome maturation in primary human macrophages

Figure 2

Co-localisation of early endosome markers with H. pylori and E. coli phagosomes. Individual panels show the amount of co-localisation between the early endosome markers Rab5 (open circle and dashed line) and EEA1 (closed circle and solid line) and H. pylori (NL101, NL103, NL106, NL107, SS1) or E. coli (ECOL) phagosomes. Time-points were 0 minutes, 15 minutes, 30 minutes, two hours and four hours following initiation of phagocytosis. Co-localisation between the bacteria (FITC) and early endosome markers (CY3) was quantified by calculating the overlap coefficients from confocal immunofluorescence images of macrophages containing approximately 100 phagosomes for each treatment, in duplicate.

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