Helicobacter pylori phagosome maturation in primary human macrophages

Table 2 The appearance of megasomes in H. pylori-infected primary human macrophages correlates with acquisition of EEA1 and Rab7 and the intracellular position of the bacteria.

*H. pylori* strain	Time after infection	EEA1 colocalisation	Rab7 colocalisation	Intracellular position	Megasomes
NL101	0 min	+	-	CS/P	-
	15 min	+	+	Cy	-
	30 min	+	+	PN	+
	2 hr	+	+	PN	+
	4 hr	+	+	PN	+
NL103	0 min	+	-	CS/P	-
	15 min	+	-	CS/P	-
	30 min	+	-	Cy	-
	2 hr	+	+	PN	+
	4 hr	+	+	PN	+
NL106	0 min	+	-	CS/P	-
	15 min	+	-	CS/P	-
	30 min	+	-	Cy	-
	2 hr	+	-	PN	+
	4 hr	+	+	PN	+
NL107	0 min	-	+	CS/P	-
	15 min	+	+	PN	+
	30 min	+	+	PN	+
	2 hr	+	+	PN	+
	4 hr	+	+	PN	+
SS1	0 min	-	-	CS/P	-
	15 min	+	+	Cy	-
	30 min	+	+	PN	+
	2 hr	+	+	PN	+
	4 hr	+	+	PN	+

H. pylori clinical strains: NL101, NL103, NL106, NL107, SS1. Time after infection of primary human macrophages: 0 minutes, 15 minutes, 30 minutes, 2 hours and 4 hours. Intracellular position of H. pylori strains in infected primary human macrophages: CS/P; cell surface/periphery, Cy: cytoplasmic, PN: peri-nuclear. Association of EEA1/Rab7 with H. pylori phagosomes and appearance of megasomes is either present (+) or absent (-).

ISSN: 1757-4749