PCR-based genotyping of Helicobacter pylori of Gambian children and adults directly from biopsy specimens and bacterial cultures

Table 4 Primers used in this study

Region	Primer	Nucleotide sequence	bp	reference
H. pylori 16sRNA	Hp1	ctg gag aga cta agc cct cc	109	[19]
	Hp2	att act gac gct gat tgt gc
cagA	cagA-F	gat aac agg caa gct ttt gag g	349	[20]
	cagA-R	ctg caa aag att gtt tgg cag a
cag empty site	Luni-1	aca ttt tgg cta aat aaa cgc tg	535	[20]
	R5280	ggt tgc acg cat ttt ccc tta atc
vacA s1 & vacA s2	Va1-F	atg gaa ata caa caa aça cac	s1 259	[20]
	Va1-R	ctg ctt gaa tgc gcc aaa c	s2 289
vacA m1a	Va3-F	ggt caa aat gcg gtc atg g	290	[20]
	Va3-R	cca ttg gta cct gta gaa ac
vacA m2	Va4-F	gga gcc cca gga aac att g	352	[20]
	Va4-R	cat aac tag cgc ctt gca c
iceA1	1048-F	gct tgt aac gat aag aaa cgc cag at	297	[20]
	1345R	gga atg agc ttg tat tta gag ccg at
iceA2	ICEA2-F	gtt ggg tat atc aca att tat	229	[20]
	ICEA2-R	ttr ccc tat ttt cta gta ggt	334

ISSN: 1757-4749