Table 2 Real-time PCR assays of the pathogen panel applied in this study.
From: Real-time PCR analysis of enteric pathogens from fecal samples of irritable bowel syndrome subjects
PCR assay (Target gene) | Sequence (5'-> 3') | Amplicon size (bp) | Ta (°C) | MgCl2 (mM) | Reference |
|---|---|---|---|---|---|
Aeromonas spp. (aerA) | F: GAGAAGGTGACCACCAAGAACA R: AACTGACATCGGCCTTGAACTC | 232 | 61 | 2 | [57] |
Bacillus cereus group (16S rRNA) | F: TCGAAATTGAAAGGCGGC R: GGTGCCAGCTTATTCAAC | 288 | 64 | 3 | [58] |
Campylobacter jejuni (hipO) | F: GACTTCGTGCAGATATGGATGCTT R: GCTATAACTATCCGAAGAAGCCATCA | 344 | 58 | 3 | [59] |
Clostridium difficile (16S rRNA) | F: TTGAGCGATTTACTTCGGTAAAGA R: CCATCCTGTACTGGCTCACCT | 157 | 58 | 3 | [55] |
Clostridium perfringens (plc) | F: AAGTTACCTTTGCTGCATAATCCC R: ATAGATACTCCATATCATCCTGCT | 283 | 61 | 4 | [60] |
Clostridium perfringens (cpe) | F: GGTTCATTAATTGAAACTGGTG R: AACGCCAATCATATAAATTACAGC | 154 | 58 | 3 | [61] |
EHEC/EPEC (eaeA) | F: ATGCTTAGTGCTGGTTTAGG R: GCCTTCATCATTTCGCTTTC | 248 | 65 | 4 | [62] |
Helicobacter pylori (16S rRNA) | F: GAAGATAATGACGGTATCTAAC R: ATTTCACACCTGACTGACTAT | 139 | 58 | 4 | [55] |
Listeria monocytogenes (iap) | F: CTAAAGCGGGAATCTCCCTT R: CCATTGTCTTGCGCGTTAAT | 174 | 61 | 4 | [63] |
Salmonella spp. (invA) | F: GTGAAATTATCGCCACGTTCGGGCAA R: TCATCGCACCGTCAAAGGAACC | 281 | 64 | 2 | [64] |
Staphylococcus aureus (nuc) | F: GCGATTGATGGTGATACGGTT R: AGCCAAGCCTTGACGAACTAAAGC | 279 | 60 | 3 | [65] |
Yersinia enterocolitica (ail) | F: GGTCATGGTGATGTTGATTACTATTCA R: CGGCCCCCAGTAATCCATAA | 90 | 58 | 4 | [66] |