Figure 2

(A) MTT assay of PBMCs indicating no cytotoxic effect upon treatment with MAP lysate (B) MTT assay of AGS cells after treatment with MAP lysate resulted in no cytotoxicity (C) MTT assay of PANC-1 cells on treatment with MAP lysate indicated no cytotoxicity at 48 hr but significant cytotoxicity was observed in PANC-1 cells at 72 hr and 96 hr. The statistical analysis between untreated and with different concentration of MAP lysate of PBMCs and AGS cells at 48 hr, 72 hr and 96 hr resulted in p > 0.05 indicating there is no statistically significant cytotoxicity upon treatment with MAP lysate when compared to untreated. The statistical analysis between untreated fractions of PANC-1 and those treated with different concentrations of MAP lysate at 48 hr resulted in p > 0.05 indicating that there is no significant cytotoxicity on treatment with MAP lysate but the statistical analysis of PANC-1 untreated and treated with different concentration of MAP lysate at 72 hr and 96 hr indicated p < 0.05 indicating significant cytotoxicity. The OD₅₇₀ values are represented as % cell viability and one of the OD₅₇₀ of the untreated cells is taken as 100% viability for each cell types in MTT assay.