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Figure 3 | Gut Pathogens

Figure 3

From: Rapid paracellular transmigration of Campylobacter jejuni across polarized epithelial cells without affecting TER: role of proteolytic-active HtrA cleaving E-cadherin but not fibronectin

Figure 3

Detection of proteolytic active HtrA multimers in different C. jejuni wild-type and mutant strains. (A) Total cell lysates of plate-grown C. jejuni 81–176 wild-type (wt), 81-176ΔhtrA, NCTC11168 wt, NCTC11168ΔhtrA and NCTC11168 htrA S197A were investigated for protease activities by casein zymography. The position of active monomeric and multimeric HtrA proteins is indicated with arrows. Active monomeric HtrA was only weakly visible as indicated. The asterisk indicates the inactive HtrA multimer formed by the S197A point mutant. (B) Total cell lysates of the indicated plate-grown wt strains were separated on non-denaturing gels. Proteolytic active HtrA bands were detected by casein zymography as described. Purified recombinant H. pylori (Hp) HtrA served as control

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Gut Pathogens

ISSN: 1757-4749