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Figure 5 | Gut Pathogens

Figure 5

From: Rapid paracellular transmigration of Campylobacter jejuni across polarized epithelial cells without affecting TER: role of proteolytic-active HtrA cleaving E-cadherin but not fibronectin

Figure 5

In vivo cleavage of E-cadherin during C. jejuni infection of non-polarised INT-407 cells and polarised MKN-28 cells. (A) INT-407 cells were infected with C. jejuni NCTC11168 wild-type (wt) strain in a time course. The Western blot shows changes of cell-associated full-length E-cadherin and reveals the generation of 90 kDa NTF domain in the supernatant over time. (B) Polarised MKN-28 cells were infected with C. jejuni 81–176 wt and htrA mutant strains in a time course. Supernatants were harvested at each time point, and the E-cadherin NTF fragment was detected using the indicated antibody. GAPDH expression levels were determined as loading control of total cellular protein

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Gut Pathogens

ISSN: 1757-4749