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Figure 6 | Gut Pathogens

Figure 6

From: Rapid paracellular transmigration of Campylobacter jejuni across polarized epithelial cells without affecting TER: role of proteolytic-active HtrA cleaving E-cadherin but not fibronectin

Figure 6

Transmigration characteristics of different bacterial pathogens across polarised MKN-28 cells using a transwell filter system. (A) MKN-28 cells were grown to reach monolayers in a transwell filter system. The cells were then differentiated and TER was allowed to establish over 14 days as indicated. (B) MKN-28 cells were then infected and CFUs of transmigrated C. jejuni wt strain 81–176 and some other indicated bacterial pathogens in a time course. Transmigrated bacteria were harvested from the bottom chambers, grown on MH plates, and CFUs were determined in triplicates. (C) TER measurement of infected MKN-28 cells during the indicated time course

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