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Figure 2 | Gut Pathogens

Figure 2

From: Distinct repeat motifs at the C-terminal region of CagA of Helicobacter pylori strains isolated from diseased patients and asymptomatic individuals in West Bengal, India

Figure 2

Western blot analysis of H. pylori strains expressing representative CagA variants. H. pylori strains were grown on brain heart infusion agar plates, harvested in PBS and subjected to SDS-PAGE followed by western blot analysis using anti-CagA antibody. Strain San 77, which carries CagA with five EPIYA motifs and gave ~810 bp amplicon in PCR using primer CAG1/CAG2 expressed CagA protein with highest molecular weight (lane 1). PCR98i and PCR24 which carry only two and single EPIYA motifs, gave ~550 bp and ~450 bp amplicon respectively in the same PCR expressed CagA proteins with much smaller size (lane 2 and 3). These two strains expressed CagA proteins, which are smaller than CagA expressed by strain San10 (lane 6) that carries 3 EPIYA motifs and gave ~642 bp amplicon, although I-27i (lane 4), which also carries two EPIYA motifsexpressed CagA protein with higher size than PCR98i (lane 2). Strain I-80, which carries no cagA gene, was used as negative control (lane 8).

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Gut Pathogens

ISSN: 1757-4749