Table 1 Primers used in this study
Primer name | Oligonucleotide sequence (5′ to 3′) |
|---|---|
For semiquantitative RT-PCR | |
rrs-F | CCAGCAGCCGCGGTAAT |
rrs-R | TTTACGCCCAGTAATTCCGATT |
ramA-F | ATTTGAATCAGCCGTTACGTATTG |
ramA-R | TGCAGGTGCCACTTGGAAT |
For quantitative RT-PCR | |
gmk-f | TTGGCAGGGAGGCGTTT |
gmk-r | GCGCGAAGTGCCGTAGTAAT |
gyrB-f | TCTCCTCACAGACCAAAGATAAGCT |
gyrB-r | CGCTCAGCAGTTCGTTCATC |
rrs-f | CCAGCAGCCGCGGTAAT |
rrs-r | TTTACGCCCAGTAATTCCGATT |
ramA-f | GCGTGAACGGAAGCTAAAAC |
ramA-r | GGCCATGCTTTTCTTTACGA |
acrB-f | TCGTGTTCCTGGTGATGTACCT |
acrB-r | AACCGCAATAGTCGGAATCAA |
mdtE-f | AGTCGCTGGATACCACCATC |
mdtE-r | GATATTACGCACGCCGATTT |
tolC-f | GCCCGTGCGCAATATGAT |
tolC-r | CCGCGTTATCCAGGTTGTTG |
flhC-f | ATATCCAGTTGGCGATGGAG |
flhC-r | TTGCTCCCAGGTCATAAACC |
hilA-f | CATGGCTGGTCAGTTGGAG |
hilA-r | CGTAATTCATCGCCTAAACG |
invF-f | TGAAAGCCGACACAATGAAAAT |
invF-r | GCCTGCTCGCAAAAAAGC |
invA-f | GGCGCCAAGAGAAAAAGATG |
invA-r | CAAATATAACGCGCCATTGCT |
sipA-f | TTTGGCTGTACGTTAGATCCGTTA |
sipA-r | CCGCCGCTTTGTCAACA |