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Figure 2 | Gut Pathogens

Figure 2

From: Quorum sensing in the probiotic bacterium Escherichia coli Nissle 1917 (Mutaflor) – evidence that furanosyl borate diester (AI-2) is influencing the cytokine expression in the DSS colitis mouse model

Figure 2

AI-1 and AI-2 production in E.coli Nissle and E.coli DH5α with the sensor bacteria E.coli DH5α [pSB403] (A), V. harveyi BB886 (B) and V. harveyi BB170 (C). Overnight cultures of E.coli Nissle and E.coli DH5α were diluted 1:100 in LB medium containing 0.5% glucose. At the given time intervals aliquots were drawn, the bacterial numbers were determined by serial dilutions (lines; right Y-axis). Bacterial supernatants were obtained by centrifugation and frozen at −80°C. The presence of AI-1 and AI-2 was determined by incubating the respective sensor bacteria with the supernatants (via the luminescence (bars) – left Y-axis).

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Gut Pathogens

ISSN: 1757-4749