Table 1 Bacterial strains and primers used
Name | Sequence (5'→3') |
|---|---|
Primer for E.coli Nissle Mutaflor | |
Mut7f | GACCAAGCGATAACCGGATG |
Mut8r | GTGAGATGATGGCCACGATT |
Primer for “Targetron”mutation: | |
IBS | AAAAAAGCTTATAATTATCCTTACTGCCCCACCGG |
GTGCGCCCAGATAGGGTG | |
EBS1d | CAGATTGTACAAATGTGGTGATAACAGATAAGTCC |
ACCGGTTTAACTTACCTTTCTTTGT | |
EBS2 | TGAACGCAAGTTTCTAATTTCGGTTGGCAGTCGAT |
AGAGGAAAGTGTCT | |
EBSuniv | CGAAATTAGAAACTTGCGTTCAGTAAAC |
RT PCR Primer: | |
GAPDHf | CCAGCCGAGCCACATCGCTC |
GAPDHr | ATGAGCCCCAGCCTTCTCCAT |
IFN-γf | TCAAGTGGCATAGATGTGGAAGAA |
IFN-γr | TGGCTCTTGCAGGATTTTCATG |
IL-6f | GAGGATACCACTCCCAACAGACC |
IL-6r | AAGTGCATCATCGTTGTTCATACA |
IL-10f | AGGCGCTGTCATCGATTTCTC |
IL-10r | TGGCCTTGTAGACACCTTGGTC |
mBD-1f | ATGAAAACTCATTACTTTCTCCTGG |
mBD-1r | ACTACTGTCAGCTCTTACAACA |
TNF-αf | GCAAGCTTCGCTCTTCTGTCTACTGAACTTCGG |
TNF-αr | GCTCTAGAATGAGATAGCAAATCGGCTGACGG |
Bacteria: | |
DH5α [pSB403] | DH5α carrying plasmid pSB403 |
E. coli Nissle 1917 | DSM6601; Serovar 06:K5:H1 |
E. coli Nissle::lux S | Nissle mutated in the lux S gene |
Vibrio harveyi BB120 | Vibrio harveyi wildtype |
Vibrio harveyi BB886 | Vibrio harveyi AI-1 Sensor |
Vibrio harveyi BB170 | Vibrio harveyi AI-2 Sensor |