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Table 4 16S rRNA gene-targeted group specific primers per bacterial group/species used in this study

From: Differences in gut microbiota composition between obese and lean children: a cross-sectional study

Target

Primer sequence (5’-3’)

Reference strains

Annealing temp (°C)

Reference

Bacteroidetes

    

Bacteroides-Prevotella-Porphyromonas spp.

GGTGTCGGCTTAAGTGCCAT

Bacteroides fragilis DSM 2151/LMG 10263

68

[31]

CGGA(C/T)GTAAGGGCCGTGC

Firmicutes

    

Staphylococcus spp.

GCGATTGATGGTGATACG

Staphylococcus aureus ATCC 29213

55

[29]

 

AGCCAAGCCTTFACGAACTAAAGC

   

Clostridium coccoides-Eubacterium rectale group (clostridial cluster XIV)

CGGTACCTGACTAAGAAGC

Ruminococcus productus DSM 2950/YIT 6141

55

[31]

 

AGTTT(C/T)ATTCTTGCGAACG

   

C. leptum group (clostridial cluster IV)

GCACAAGCAGTGGAGT

Faecalibacterium prausnitzii YIT 6174

50

[30]

 

TTCCTCCGTTTTGTCAA

   

Lactobacillus spp.

AGCAGTAGGGAATCTTCCA

Lactobacillus acidophilus LMG 9433

58

[31]

 

CACCGCTACACATGGAG

   

Actinobacteria

    

Bifidobacterium spp.

TCGCGTC(C/T)GGTGTGAAAG

Bifidobacterium longum DSM 20219/YIT 4021

58

[31]

CCACATCCAGC(A/G)TCCAC