From: Differences in gut microbiota composition between obese and lean children: a cross-sectional study
Target | Primer sequence (5’-3’) | Reference strains | Annealing temp (°C) | Reference |
---|---|---|---|---|
Bacteroidetes | Â | Â | Â | Â |
Bacteroides-Prevotella-Porphyromonas spp. | GGTGTCGGCTTAAGTGCCAT | Bacteroides fragilis DSM 2151/LMG 10263 | 68 | [31] |
CGGA(C/T)GTAAGGGCCGTGC | ||||
Firmicutes | Â | Â | Â | Â |
Staphylococcus spp. | GCGATTGATGGTGATACG | Staphylococcus aureus ATCC 29213 | 55 | [29] |
 | AGCCAAGCCTTFACGAACTAAAGC |  |  |  |
Clostridium coccoides-Eubacterium rectale group (clostridial cluster XIV) | CGGTACCTGACTAAGAAGC | Ruminococcus productus DSM 2950/YIT 6141 | 55 | [31] |
 | AGTTT(C/T)ATTCTTGCGAACG |  |  |  |
C. leptum group (clostridial cluster IV) | GCACAAGCAGTGGAGT | Faecalibacterium prausnitzii YIT 6174 | 50 | [30] |
 | TTCCTCCGTTTTGTCAA |  |  |  |
Lactobacillus spp. | AGCAGTAGGGAATCTTCCA | Lactobacillus acidophilus LMG 9433 | 58 | [31] |
 | CACCGCTACACATGGAG |  |  |  |
Actinobacteria | Â | Â | Â | Â |
Bifidobacterium spp. | TCGCGTC(C/T)GGTGTGAAAG | Bifidobacterium longum DSM 20219/YIT 4021 | 58 | [31] |
CCACATCCAGC(A/G)TCCAC |