Table 4 16S rRNA gene-targeted group specific primers per bacterial group/species used in this study
From: Differences in gut microbiota composition between obese and lean children: a cross-sectional study
| Target | Primer sequence (5’-3’) | Reference strains | Annealing temp (°C) | Reference |
|---|---|---|---|---|
| Bacteroidetes | ||||
| Bacteroides-Prevotella-Porphyromonas spp. | GGTGTCGGCTTAAGTGCCAT | Bacteroides fragilis DSM 2151/LMG 10263 | 68 | [31] |
| CGGA(C/T)GTAAGGGCCGTGC | ||||
| Firmicutes | ||||
| Staphylococcus spp. | GCGATTGATGGTGATACG | Staphylococcus aureus ATCC 29213 | 55 | [29] |
| AGCCAAGCCTTFACGAACTAAAGC | ||||
| Clostridium coccoides-Eubacterium rectale group (clostridial cluster XIV) | CGGTACCTGACTAAGAAGC | Ruminococcus productus DSM 2950/YIT 6141 | 55 | [31] |
| AGTTT(C/T)ATTCTTGCGAACG | ||||
| C. leptum group (clostridial cluster IV) | GCACAAGCAGTGGAGT | Faecalibacterium prausnitzii YIT 6174 | 50 | [30] |
| TTCCTCCGTTTTGTCAA | ||||
| Lactobacillus spp. | AGCAGTAGGGAATCTTCCA | Lactobacillus acidophilus LMG 9433 | 58 | [31] |
| CACCGCTACACATGGAG | ||||
| Actinobacteria | ||||
| Bifidobacterium spp. | TCGCGTC(C/T)GGTGTGAAAG | Bifidobacterium longum DSM 20219/YIT 4021 | 58 | [31] |
| CCACATCCAGC(A/G)TCCAC |