Skip to main content
Figure 6 | Gut Pathogens

Figure 6

From: In vitro evaluation of anti-infective activity of a Lactobacillus plantarum strain against Salmonella enterica serovar Enteritidis

Figure 6

Confocal images of Salmonella invasion (A-D) and Expression of hilA gene by RT-PCR (E). Confocal images were taken at 63X magnification using Leica CLSM. The membrane of HCT-116 cell lines were stained with plasma red dye and S. Enteritidis was tagged with GFP. The KSBT 56 strain was observed in phase contrast. A. S. Enteritidis invasion into HCT-116 in the absence of KSBT 56 strain. B. S. Enteritidis coinfected with KSBT 56 strain into HCT-116 cell line shows reduced invasion of S. Enteritidis. C. Delayed addition of S. Enteritidis after addition of KSBT 56 strain by 1 h further reduces the invasion of Salmonella into the cell lines. D. Merged image of panel C with phase contrast shows KSBT 56 adhering to HCT-116. E. RT-PCR analysis of hilA gene of S. Enteritidis grown in increasing concentration of CFCS of KSBT 56 strain. L. plantarum MTCC 1407 is a reference strain. The fold change in the expression of hilA gene is compared to S. Enteritidis WT (Untreated). SEn refers to S. Enteritidis and LP refers to L. plantarum MTCC 1407.

Back to article page