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Figure 1 | Gut Pathogens

Figure 1

From: Uropathogenic specific protein gene, highly distributed in extraintestinal uropathogenic Escherichia coli, encodes a new member of H-N-H nuclease superfamily

Figure 1

Construction of the E. coli strain overproducing recombinant Usp. (A) Comparison of colony formation by E. coli DH5α (left panel) or E. coli BL21(DE3) (right panel) after transforming with plasmids encoding Usp alone or Usp together with OrfUs. The plasmids encoding Usp alone or Usp together with OrfUs (for details, see Table 1) were transformed into E. coli DH5α or E. coli BL21(DE3) and selected on kanamycin containing LB agar plate. The number of colony observed on the Φ100 mm plate by each transformed E. coli after overnight incubation at 37°C is indicated on y-axis. Data are means ± SD of values from three experiments. (B) CBB staining analysis of the expression of Usp and OrfUs in transformed E. coli BL21(DE3). The E. coli BL21(DE3) cells harboring plasmids indicated were cultured for 3 hour, and then the expression of recombinant proteins was induced by IPTG. Cells were harvested 6 hour post induction. Protein expressions were analyzed by subjecting whole cell protein extracts to SDS-PAGE followed by CBB staining. Molecular weights are estimated based on deduced amino acid sequence of each protein.

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Gut Pathogens

ISSN: 1757-4749