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Figure 2 | Gut Pathogens

Figure 2

From: Uropathogenic specific protein gene, highly distributed in extraintestinal uropathogenic Escherichia coli, encodes a new member of H-N-H nuclease superfamily

Figure 2

Purification and molecular weight analysis of free recombinant Usp. (A) Purity analysis of purified free recombinant Usp. Free Usp was purified from E. coli BL21(DE3) over-expressing Usp and OrfU1-His as described under Methods. Lane 1, marker; lane 2, total cell proteins extracted from E. coli BL21(DE3) cells over-expressing Usp and OrfU1-His and lane 3, free Usp eluted with 6 M guanidine HCl. (B) Elution profile of purified free recombinant Usp from gel filtration column. Free Usp which was dialyzed against 50 mM sodium phosphate buffer was separated by gel filtration column. Opened triangles indicate elution positions of molecular weight markers. Calculated molecular weight was indicated on each peak.

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