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Table 2 Primers used in this study

From: Uropathogenic specific protein gene, highly distributed in extraintestinal uropathogenic Escherichia coli, encodes a new member of H-N-H nuclease superfamily

Primers

Sequence

uspF1

5′-aacatatg ctactgttcccgagtag-3′

uspR1

5′-aactcgag tctcctgtagtgaatttc-3′

orfu3R1

5′-aactcgag ttctgaatctttgaacaaag-3′

orfu2R1

5′-aactcgag tctactttgtttagagtctttaaac-3′

orfu1R1

5′-aactcgag tttagagtctttaaacaaggg-3′

HH314AAF

5′-gttaagaaattccagatagc tgc tgtagttgctatagaacatgg-3′

HH314AAR

5′-ccatgttctatagcaactacagc agc tatctggaatttcttaac-3′

N330AF

5′-gtggagtgtatgatattgatgc ttttaggattgttacgcccc-3′

N330AR

5′-ggggcgtaacaatcctaaaagc atcaatatcatacactccac-3′

H339AF

5′-gattgttacgccccgactagc tgatgaaattcactacagg-3′

H339AR

5′-cctgtagtgaatttcatcagc tagtcggggcgtaacaatc-3′

  1. Restriction enzyme sites are underlined and sites of mutations in mutagenic primers are underlined and bold.