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Figure 3 | Gut Pathogens

Figure 3

From: Exploring the role of Mycobacterium avium subspecies paratuberculosis in the pathogenesis of type 1 diabetes mellitus: a pilot study

Figure 3

Nested PCR detection of MAP DNA in blood culture. Two rounds of nested PCR consisting of first set using P90/91 oligonucleotide primers and second round set using AV1/AV2 oligonucleotides primers. PCR product of 298 base pair indicates positive result for MAP. M: Molecular weight marker in base pair. =: Negative control for second round of PCR. -: Negative control for first round of PCR. TE: Negative control for DNA extraction. +: DNA template from MAP strain UCF4 as a positive control. Lanes 1 to 18: correspond with the code of each blood sample used in this study. Codes with a prime (‘) indicate that 1 uL of PCR product from P90/91 PCR set was used as template for the second AV1/AV2 round of PCR whereas codes without prime indicate that 23 uL of PCR product from P90/91 PCR set was used as template.

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