Figure 2

Increased occludin cleavage in jejunum samples of Giardia -infected mice during parasite colonization and post-clearance. The tight junctional integrities of intestinal tissues of Giardia-infected mice and PBS control groups were examined on PI days 7 and 35. (A) Western blots depicting occludin cleavage in the jejunal mucosa of Giardia-infected mice, but not saline controls, on PI days 7 and 35. Representative blots from at least two individual experiments. n = 4/group. *P < 0.05 vs. PBS. (B) No signs of occludin cleavage were observed in the colonic mucosa of infected mice or saline controls on PI days 7 and 35. Representative blots from at least two individual experiments. n = 4/group. (C) Luminal-to-serosal dextran flux in jejunal tissues of infected mice on PI day 7 (a) and day 35 (b). n = 6/group. *P < 0.05 vs. PBS. (D) Luminal-to-serosal dextran flux in colonic tissues of infected mice on PI day 7 (a) and day 35 (b). n = 6/group. *P < 0.05 vs. PBS.