Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines

Table 2 E. coli adhesion patterns: cell line, tissue, host cell specificity

Adhesion pattern specificity	Cell lines	Diffusely distributed	Micro-colonies	Chains	Various pattern
Cell line	Caco-2	28	6	1	-
	IPEC-J2	12	3	-	-
	5637	10	-	-	-
	PK-15	1	-	-	-
Tissue	Intestinal epithelium (Caco-2, IPEC-J2)	16	9	-	1
Tissue	Urinary epithelium (5637, PK-15)	1	-	-	-
Host cell	Human (Caco-2, 5637)	9	8	-	1
Host cell	Porcine (IPEC-J2, PK-15)	3	3	-	-
Unspecific	All cell lines	8	20	3	12
	Other groups of cell lines	21	8	2	8

Classified are isolates which adhered with a distinct pattern (diffusely distributed, microcolonies, chains) to at least one cell line (n = 172), and isolates with different adhesion patterns to the four cell lines (various pattern, n = 22). Non-adherent isolates (n = 88) were excluded. Most distinct pattern-forming isolates were diffusely distributed on at least one cell line (n = 109) followed by microcolony forming isolates (n = 57). “Other groups of cell lines” includes isolates with adhesion patterns on the following cell lines: 1) Caco-2 and 5637 and IPEC-J2; 2) Caco-2 and 5637 and PK-15; 3) Caco-2 and IPEC-J2 and PK-15; 4) 5637 and IPEC-J2 and PK-15; 5) Caco-2 and PK-15; 6) 5637 and IPEC-J2.

ISSN: 1757-4749