Table 2 Used primers and probes
Primer and probe | Sequence (5′-3′) | Reference |
|---|---|---|
Bacteroides sp. | [14] | |
Forward | TGGTAGTCCACACAGTAAACGATGA | |
Reverse | CGTACTCCCCAGGTGGAATACTT | |
Probe | GTTTGCCATATACAGTAAGCGGCCAAGCG | |
Bifidobacterium sp . | [15] | |
Forward | CGGGTGAGTAATGCGTGACC | |
Reverse | TGATAGGACGCGACCCCA | |
Probe | CTCCTGGAAACGGGTG | |
Clostridium leptum | [15] | |
Forward | CCTTCCGTGCCGSAGTTA | |
Reverse | GAATTAAACCACATACTCCACTGCTT | |
Probe | CACAATAAGTAATCCACC | |
Clostridium coccoides | [15] | |
Forward | GACGCCGCGTGAAGGA | |
Reverse | AGCCCCAGCCTTTCACATC | |
Probe | CGGTACCTGACTAAGAAG | |
Enterobactericaea | This study | |
Forward | GCGGTAGCACAGAGAGCTT | |
Reverse | GGCAGTTTCCCAGACATTACTCA | |
Probe | CCGCCGCTCGTCACC | |
Lactobacillus sp. | This study | |
Forward | GCTAGGTGTTGGAGGGTTTCC | |
Reverse | CCAGGCGGAATGCTTAATGC | |
Probe | TCAGTGCCGCAGCTAA | |
Streptococcus sp. mainly Str. mitis- group* | [13] | |
Forward | CCAGCAGCCGCGGTAATA | |
Reverse | CCTGCGCTCGCTTTACG | |
Probe | ACGCTCGGGACCTACG | |
Universal | [16] | |
Forward | TGGAGCATGTGGTTTAATTCGA | |
Reverse | TGCGGGACTTAACCCAACA | |
Probe | CACGAGCTGACGACA[A/G]CCATGCA | |