Table 1 Strains and plasmids used in this study

Serovar Enteritidis phage type 8 E2627

Isolated from an egg-associated outbreak in the United States

[26]

∆stdA

Mutant of serovar Enteritidis in which stdA was deleted using lambda Red; Kn^r

This study

∆stdA/ pBRstdA

∆stdA complemented with a copy of the stdA gene via pBR322; Kn^r, Tc^r

This study

E. coli

DH5α

Used for recombinant DNA methods

Lab stock

Plasmids

pKD46

lambda Red recombinase genes; Ap^r

Lab stock

pBR322

Ap^r Tc^r

Lab stock

pKD4

Kn^r gene cassette

Lab stock

pBRstdA

stdA gene cloned into pBR322 at the ScaI site

This study