Table 2 Sequence and purpose of primers used in this study

LF:5′-AAAGGACATATTATCTATGCGTAATAAAATAATACTTGCCTGTGTAGGCTGGAGCTGCTT-3′

Forward primer for amplification of the Kn^R gene cassette and upstream stdA flanking sequence

LR:5′-CCGTGGACGGCTTCTCCCTGTCGTTATTTACCGCGTGAAACATATGAATATCCTCCTTAG-3′

Reverse primer for amplification of the Kn^R gene cassette and downstream stdA flanking sequence

F₂:5′-CATCACCAACTCACCCTGTG-3′

Forward primer for amplification of the stdA gene

R₂:5′-CTGAGGTATCTGCTGTGCCA-3′

Reverse primer for amplification of the stdA gene

F₃:5′-ATTCATATGGTGCTTCGTTTAACACC-3′

Forward primer for amplification of stdA for complementation

R₃:5′-AGACTCGAGTCACAGGTATTTCAGG-3′

Reverse primer for amplification of stdA for complementation

K₃: 5′-AAAGCCACGTTGTGTCTA-3′

Forward primer for amplification of the Kn^r gene cassette

K₅: 5′- CGCTGAGGTCTGCCTCGT-3′

Reverse primer for amplification of the Kn^r gene cassette