Table 1 Primers and amplification conditions used in this study
From: The virulence phenotypes and molecular epidemiological characteristics of Vibrio fluvialis in China
Primer | Sequences (5’-3’) | Target size (bp) | Annealing temp. (°C) | Reference |
|---|---|---|---|---|
toxR-F | GACCAGGGCTTTGAGGTGGACGAC | 217 | 65 | [32] |
toxR-R | AGGATACGGCACTTGAGTAAGACTC | Â | Â | Â |
VFLU-F | ATAAAGTGAAGAGATTCGTACC | 278 | 60 | [33] |
VFLU-R | GTATTCCTGAATGGAATACAC | Â | Â | Â |
Int IV-F | AACACCGCTTGCACCTCTAT | 525 | 53 | [34] |
Int IV-R | TGTATGCGCTTGAGAGTCC | Â | Â | Â |
stn-F | GGTGCAACATAATAAACAGTCAACAA | 375 | 53 | [44] |
stn-R | TAGTGGTATGCGTTGCCAGC | Â | Â | Â |
sul II-F | AGGGGGCAGATGTGATCGAC | 606 | 55 | [35] |
sul II-B | TGTGCGGATGAAGTCAGCTCC | Â | Â | Â |
vfh-F | GCGCGTCAGTGGTGGTGAAG | 800 | 61 | This study |
vfh-R | TCGGTCGAACCGCTCTCGCTT | Â | Â | Â |
hupO-F | ATTACGCACAACGAGTCGAAC | 600 | 56 | This study |
hupO-R | ATTGAGATGGT AAACAGCGCC | Â | Â | Â |
vfpA -F | TACAACGTCAAGTTAAAGGC | 1790 | 55 | This study |
vfpA -R | GTAGGCGCTGTAGCCTTTCA | Â | Â | Â |
arc- F | AGTTTATGCGTCTGGCTTG | 3427 | 56 | This study |
arc-R | ATGAGTAAGTTATACGTAGG | Â | Â | Â |
arc-rev | GCTTCGGCCCACATAATAA (paired with arc-F) | 2170 | 56 | This study |
arc-ck-up | TTACCACCTAATGCGACGA (paired with arc-R) | 1235 | 56 | This study |