Detection, identification and quantification of Campylobacter jejuni, coli and lari in food matrices all at once using multiplex qPCR

Table 2 PCR primers and probes in this assay

Species	Target/GeneBank ID	Primer/probe	DNA sequence 5’→3’	Position within target	Amplicon (bp)	Reference
C. jejuni	hip O^a NC_002163.1	Forward	TGCACCAGTGACTATGAATAACGA	809-832	124
		Reverse	TCCAAAATCCTCACTTGCCATT	911-932		He et al., 2010 [28]
		Probe	JOE-TTGCAACCTCACTAGCAAAATCCACAGCT-Eclipse	836-864
C. coli	gly A^b AF136494.1	Forward	CATATTGTAAAACCAAAGCTTATCGTG	271-297	133
		Reverse	AGTCCAGCAATGTGTGCAATG	384-404		LaGier et al., 2004* [18]
		Probe	FAM-TAAGCTCCAACTTCATCCGCAATCTCTCTAAATTT- Eclipse	337-371		LaGier et al., 2004* [18]
C. lari	pep T^c NC_012039.1	Forward	TTAGATTGTTGTGAAATAGGCGAGTT	519-544	86
C. lari	pep T^c NC_012039.1	Reverse	TGAGCTGATTTGCCTATAAATTCG	581-604	86	He et al., 2010* [28]
		Probe	CY5-TGAAAATTGGAAdC GdC AGGTG-BHQ	551-570

ISSN: 1757-4749