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Table 4 Comparison of results for singleplex qPCR and optimized multiplex qPCR with pure cultures

From: Detection, identification and quantification of Campylobacter jejuni, coli and lari in food matrices all at once using multiplex qPCR

Platform

Singleplex

Multiplex

Strain

Efficiency (%)

Slope

y-intercept

R2

Efficiency (%)

Slope

y-intercept

R2

C. jejuni CCM 6212

84.560

−3.757

41.308

0.999

90.848

−3.565

42.227

0.998

C. coli CCM 6211

89.235

−3.610

37.393

1.000

96.973

−3.399

40.040

0.998

C. lari CCM 4897

77.930

−3.996

40.234

0.993

92.888

−3.506

39.548

0.998

  1. R2 correlation coefficient.