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Table 4 Comparison of results for singleplex qPCR and optimized multiplex qPCR with pure cultures

From: Detection, identification and quantification of Campylobacter jejuni, coli and lari in food matrices all at once using multiplex qPCR

Platform Singleplex Multiplex
Strain Efficiency (%) Slope y-intercept R2 Efficiency (%) Slope y-intercept R2
C. jejuni CCM 6212 84.560 −3.757 41.308 0.999 90.848 −3.565 42.227 0.998
C. coli CCM 6211 89.235 −3.610 37.393 1.000 96.973 −3.399 40.040 0.998
C. lari CCM 4897 77.930 −3.996 40.234 0.993 92.888 −3.506 39.548 0.998
  1. R2 correlation coefficient.