Figure 2From: A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticlesFlow chart of FNP-ELISA. Preparation of IMMPs and B-GNPs (part A); (part B) comprises five steps, namely, magnetic separation of target cells (step 1), conjugation of B-GNPs (step 2), removal of free B-GNPs (step 3), conjugation of Strep-HRP (step 4), and removal of free Strep-HRP (step 5); and FNP-ELISA detection (part C).Back to article page