Table 2 Primers
From: Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance
Primer name | Primer sequence (5' to 3') | Length | Characteristics | |
|---|---|---|---|---|
ESA_02131 | F | CATCGGCCGACAGGCCAGTCAATGAATGATGC | 32 | Eag1cut site |
R | CATTCTAGAGAGTACAACGGAATGCGGGG | 29 | Xba1 cut site | |
ESA_01706 | F | CATTCTAGAGTCGGGCGGCTCTTTATCTGG | 30 | Xba1 cut site |
R | CATGGATCCTTGACCAGATGACGCAGTCTTTC | 32 | BamH1 cut site | |
ESA_04214 | F | CATGAATTCGTCTCTTTCTGTGCCAACTATCTGC | 34 | EcoR1 cut site |
R | CATTCTAGACTACCTGACGCGTACCCTGTATATC | 34 | Xba1 cut site | |
ESA_pESA3p05450 | F | CATGAATTCATCATCTCTACACGCTGCCTTCTG | 33 | EcoR1 cut site |
R | CATTCTAGATCTCCACCTGCGCCTCTATC | 29 | Xba1 cut site | |
ESA_01226 | F | CATGAATTCCAGTGCGCCGGAGCTTTTCG | 29 | EcoR1 cut site |
R | CATTCTAGAGGGCTGTCGGTTGACGAAATTAAAC | 34 | Xba1 cut site | |
ESA_00673 | F | CATGAATTCTAAAAGCGAAATCCTCCCGTACTGGC | 35 | EcoR1 cut site |
R | CATGGATCCCCTGCAAAGCATCGCCGATTACC | 32 | BamH1 cut stie | |
ESA_03328 | F | CATTCTAGATCGCTATCGCTGACCGTGAAATG | 32 | Xba1 cut site |
R | CATGGATCCTGCTGAACGAACAGTATGGCCG | 31 | BamH1 cut site | |
pUC18 MCS Check | F | CATTAG CTC ACT CAT TAG GCA CC | 20 | pUC18 insert check |
R | CATTGT AAA ACG ACG GCC AGT G | 19 | pUC18 insert check | |
RTESA_02131 | F | GCTGGCGTGTATCGGTCT | 18 | Probe 31 |
R | CTC GGC ATA TAA GTA AGC AGC AT | 23 | Probe 31 | |
RTESA_01706 | F | CGA CGG TCA TTC TGC TCA C | 19 | Probe 32 |
R | AGC AGG CCA ATC TGA TGG TA | 20 | Probe 32 | |
RTESA_04214 | F | GCA TAA ACG CGC CCT GTA | 18 | Probe 18 |
R | TTA GCG AAG AAG AAG CCG ATA | 21 | Probe 18 | |
RTESA_01226 | F | GGG TAT CAG GTG GCA AGC | 18 | Probe 70 |
R | GTA AAT CGC GAC CGT ATG C | 19 | Probe 70 | |
RTESA_00673 | F | CCG GAC AGA TAA ACC GTC AC | 20 | Probe 11 |
R | GAC GAG GCA CCG ACA ATC | 18 | Probe 11 | |
RTESA_03328 | F | CGT GCC GTT CGT AAT GGT | 18 | Probe 29 |
R | AAC GAA ATG CCG GTA AAG C | 19 | Probe 29 | |
RTESA_pESA3p05450 | F | TGG TGG CGA TTT CCA ACT | 18 | Probe 71 |
R | CCC GAA ACG GTC AGA AAG | 18 | Probe 71 | |
RT16S | F | TGT AGC GGT GAA ATG CGT AG | 20 | Probe 65 |
R | AGC GTC AGT CTT CGT CCA G | 19 | Probe 65 |