Analysis of the role of the Cronobacter sakazakii ProP homologues in osmotolerance

Table 3 Molecular and physiological comparison of each ProP homologue

Gene locus tag	Identity (%)	Fold increase in mRNA transcript	Complex media		Defined media supplemented with proline		Defined media supplemented with glycine betaine
			Maximum OD @ 600 nm	Growth rate (hr^-1)	Maximum OD @ 600 nm	Growth rate (hr^-1)	Maximum OD @ 600 nm	Growth rate (hr^-1)
ESA_02131	90	19.472	0.668	0.013	0.219	0.007	0.585	0.017
ESA_01706	40	2.854	0.259	0.000	0.205	0.008	0.358	0.011
ESA_04214	33	46.851	0.240	0.001	0.178	0.000	0.240	0.000
ESA_03328	30	1.598	0.255	0.003	0.261	0.021	0.397	0.021
ESA_00673	29	5.193	0.505	0.013	0.125	0.000	0.273	0.000
ESA_pESA3p05450	29	4.327	0.291	0.003	0.170	0.003	0.235	0.002

The % identity values were obtained in a previous study[11] and determined the homology of the above C. sakazakii putative proP genes relative to the well characterised E. coli proP. Growth rate, OD and transcription values shown here were obtained when growth was monitored in 6% added NaCl.

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