Clinical conditions and small intestinal histopathology following ileitis induction upon peroral T. gondii infection. In order to induce acute ileitis, C57BL/6 wildtype (WT; black circles) and TLR-9 deficient (TLR-9-/-; white circles) mice were perorally infected with 100 cysts of T. gondii ME49 strain on day 0. (A) Relative loss of body weights between day 7 following ileitis induction (ILE) and day 0 (naïve mice; N) were determined (in %). Furthermore, (B) histopathological mucosal changes were assessed in ileal paraffin sections from respective mice seven days following peroral T. gondii infection applying a standardized histopathological score. (C) T. gondii DNA was determined in ex vivo ileal biopsies at day 7 p.i.. Naïve mice served as negative controls (N). Numbers of analyzed mice are given in parentheses. Medians (black bars) and significance levels (P- values) determined by Mann–Whitney-U test are indicated. Data shown are pooled from three independent experiments.