Pro-inflammatory cytokine responses in mesenteric lymph nodes following ileitis induction. (A) IFN-γ, B) TNF-α, and (C) nitric oxide protein concentrations were determined in ex vivo biopsies of mesenteric lymph nodes (MLN) derived from C57BL/6 wildtype (WT; black circles) and TLR-9 deficient (TLR-9-/-; white circles) mice seven days following ileitis induction (ILE). Naïve mice served as negative controls (N). Numbers of analyzed mice (in parentheses), means (black bars) and levels of significance (P-values) as compared to the respective groups (determined by Mann–Whitney-U test) are indicated. Data shown were pooled from three independent experiments.