Role of Campylobacter jejuni gamma-glutamyl transpeptidase on epithelial cell apoptosis and lymphocyte proliferation

Table 1 Distribution of cell cycle phases in lymphocytes cultured with C. jejuni GGT

	Cell cycle phase
	G0/1	S	G2/M
Ly	99.0 +/- 0.2	0.6 +/- 0.2	0.2 +/- 0.1
Ly + PHA/IL-2	69.9 +/- 0.8	18.0 +/- 0.7	11.1 +/- 1.3
Ly + PHA/IL-2 + acivicin	71.3 +/- 2.0	18.0 +/- 0.7	8.0 +/- 2.1
Ly + PHA/IL-2+ *C. jejuni* GGT	97.5 +/- 0.6	1.2 +/- 0.5	1.0 +/- 0.1
Ly + PHA/IL-2+ *C. jejuni* GGT + acivicin	68.6 +/- 1.9	17.7 +/- 0.9	12.3 +/- 0.7
Ly + PHA/IL-2+ heat inactivated *C. jejuni* GGT	71.5 +/- 1.0	16.8 +/- 2.0	9.6 +/- 0.8

Flow cytometry study by Nicoletti assay after 4 days of culture with C. jejuni GGT (10 ng/mL). Percentage of cells in G0/G1, S and G2/M phase depending on growth conditions. These data are consistent with the results obtained during a manipulation performed in triplicate representative of the results for two independent manipulations. (Ly: lymphocytes, PHA: phyto hemagglutinin, IL-2: interleukin 2).