A2146G and A2147G mutations in the 23S rRNA gene identified by illumina sequencing. The aligned results of sequencing reads of two CLR-resistant H. pylori strains (S17 and S26) are shown. Along the top, reference nucleotide sequences of NC_000915 (ATCC26695), and consensus nucleotide sequences of each CLR-resistant strain are shown (black). The lower portion of the sequence viewer shows mapped sequencing reads (yellow and blue). In 479 reads from S17 strain (left), reference nucleotide A was changed to G at position 2146 in 23S rRNA (bold faces). Similarly, reference nucleotide A was changed to G at position 2147 in 23S rRNA in 485 reads from S26 (right).