Table 1 Bacterial strains and plasmids
From: The role of the Cronobacter sakazakii ProP C-terminal coiled coil domain in osmotolerance
Strain or plasmid | Relevant genotype or characteristics | Source or reference |
|---|---|---|
Plasmids | ||
pUC18 | Ampr, lacZ', pMB9 replicon | [9] |
pUC18: ESA_02131 | pUC18 harboring ESA_02131 gene under control of the native promoter | [7] |
pUC18: ESA_01706 | pUC18 harboring ESA_01706 gene under control the native promoter | [7] |
pUC18: ESA_01706CTE | pUC18 harboring chimeric ESA_01706 with fused C-terminal extension (ESA_02131) under control of the native promoter | This work |
Strains | ||
Cronobacter sakazakii BAA-894 | C.sakazakii strain isolated from powdered formula associated with neonatal intensive care unit | [10] |
Escherichia coli DH5α | Intermediate cloning host.supE44 ΔlacU169(80lacZΔM15)R17 recA1 endA1 gyrA96 thi-1 relA1 | Invitrogen |
MKH13 | MC4100Δ(putPA)101Δ(proP)2Δ(proU) | [11] |
MKH13 pUC18:ESA_02131+ | Host strain harbouring pUC18: ESA_02131 plasmid. Ampr | [7] |
MKH13 pUC18:ESA_01706+ | Host strain harbouring pUC18: ESA_01706 plasmid. Ampr | [7] |
MKH13 pUC18:ESA_01706CTE | Host strain harbouring pUC18: ESA_01706CTE plasmid. Ampr | This work |