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Table 1 Bacterial strains and plasmids

From: The role of the Cronobacter sakazakii ProP C-terminal coiled coil domain in osmotolerance

Strain or plasmid Relevant genotype or characteristics Source or reference
Plasmids   
pUC18 Ampr, lacZ', pMB9 replicon [9]
pUC18: ESA_02131 pUC18 harboring ESA_02131 gene under control of the native promoter [7]
pUC18: ESA_01706 pUC18 harboring ESA_01706 gene under control the native promoter [7]
pUC18: ESA_01706CTE pUC18 harboring chimeric ESA_01706 with fused C-terminal extension (ESA_02131) under control of the native promoter This work
Strains   
Cronobacter sakazakii BAA-894 C.sakazakii strain isolated from powdered formula associated with neonatal intensive care unit [10]
Escherichia coli DH5α Intermediate cloning host.supE44 ΔlacU169(80lacZΔM15)R17 recA1 endA1 gyrA96 thi-1 relA1 Invitrogen
MKH13 MC4100Δ(putPA)101Δ(proP)2Δ(proU) [11]
MKH13 pUC18:ESA_02131+ Host strain harbouring pUC18: ESA_02131 plasmid. Ampr [7]
MKH13 pUC18:ESA_01706+ Host strain harbouring pUC18: ESA_01706 plasmid. Ampr [7]
MKH13 pUC18:ESA_01706CTE Host strain harbouring pUC18: ESA_01706CTE plasmid. Ampr This work
  1. Ampr. This strain is resistant to ampicillian.