Table 2 Primers
From: The role of the Cronobacter sakazakii ProP C-terminal coiled coil domain in osmotolerance
Primer name | Primer sequence (5' to 3') | Length | Characteristics | |
|---|---|---|---|---|
ESA_02131 | F | CATCGGCCGACAGGCCAGTCAATGAATGATGC | 32 | EagI cut site |
R | CATTCTAGAGAGTACAACGGAATGCGGGG | 29 | XbaI cut site | |
ESA_01706 | F | CATTCTAGAGTCGGGCGGCTCTTTATCTGG | 30 | XbaI cut site |
R | CATGGATCCTTGACCAGATGACGCAGTCTTTC | 32 | BamHI cut site | |
Chimeric-01706 | F | AATAAGCTTGTGGCTTTTTATGCCGGGCTGC | 31 | HindIII cut site |
R | CAGGCCAGTAATCAGCGCCGCGCCCATGAC | 30 | 3' SOEing overhang | |
Chimeric-02131CTE | F | CGCGGCGCTGATTACTGGCCTGACGATGAAAG | 32 | 5' SOEing overhang |
R | AATGGATCCTTACTCGTTAATACGAGGATGCTGG | 34 | BamHI cut site | |
pUC18 MCS Check | F | CATTAGCTCACTCATTAGGCACC | 23 | pUC18 insert check |
R | CATTGTAAAACGACGGCCAGTG | 22 | pUC18 insert check |