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Table 1 Primers correspond to VP7 and VP4 genes for rotavirus genotyping

From: Genotyping and clinical factors in pediatric diarrhea caused by rotaviruses: one-year surveillance in Surabaya, Indonesia

Primer Sequence (5′ – 3′) Position PCR product (bp)
G-typing (VP7) first amplification
  VP7-F ATG TAT GGT ATT GAA TAT ACC AC nt 51-71 881
  VP7-R AAC TTG CCA CCA TTT TTT CC nt 914-932  
G-typing (VP7) second amplification
 G1 aBT1 CAA GTA CTC AAA TCA ATG ATG G nt 314-335 618
 G2 aCT2 CAA TGA TAT TAA CAC ATT TTC TGT G nt 411-435 521
 G3 mG3 ACG AAC TCA ACA CGA GAG G nt 250-269 682
 G4 aDT4 CGT TTC TGG TGA GGA GTT G nt 480-498 452
 G8 aAT8 GTC ACA CCA TTT GTA AAT TCG nt 178-198 754
 G9 mG9 CTT GAT GTG ACT AYA AAT AC nt 757-776 179
P-typing (VP4) first amplification
  VP4-F TAT GCT CCA GTN AAT TGG nt 132-149 663
  VP4-R ATT GCA TTT CTT TCC ATA ATG nt 775-795  
P-typing (VP4) second amplification
 P[4] 2T-1 CTA TTG TTA GAG GTT AGA GTC nt 474–494 362
 P[6] 3T-1 TGT TGA TTA GTT GGA TTC AA nt 259–278 146
 P[8] 1T-1 TCT ACT TGG ATA ACG TGC nt 339 –356 224
 P[9] 4T-1 TGA GAC ATG CAA TTG GAC nt 385–402 270
 P[10] 5T-1 ATC ATA GTT AGT AGT CGG nt 575–594 462
  1. References: [35].