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Table 1 Primers correspond to VP7 and VP4 genes for rotavirus genotyping

From: Genotyping and clinical factors in pediatric diarrhea caused by rotaviruses: one-year surveillance in Surabaya, Indonesia

Primer

Sequence (5′ – 3′)

Position

PCR product (bp)

G-typing (VP7) first amplification

 

VP7-F

ATG TAT GGT ATT GAA TAT ACC AC

nt 51-71

881

 

VP7-R

AAC TTG CCA CCA TTT TTT CC

nt 914-932

 

G-typing (VP7) second amplification

 G1

aBT1

CAA GTA CTC AAA TCA ATG ATG G

nt 314-335

618

 G2

aCT2

CAA TGA TAT TAA CAC ATT TTC TGT G

nt 411-435

521

 G3

mG3

ACG AAC TCA ACA CGA GAG G

nt 250-269

682

 G4

aDT4

CGT TTC TGG TGA GGA GTT G

nt 480-498

452

 G8

aAT8

GTC ACA CCA TTT GTA AAT TCG

nt 178-198

754

 G9

mG9

CTT GAT GTG ACT AYA AAT AC

nt 757-776

179

P-typing (VP4) first amplification

 

VP4-F

TAT GCT CCA GTN AAT TGG

nt 132-149

663

 

VP4-R

ATT GCA TTT CTT TCC ATA ATG

nt 775-795

 

P-typing (VP4) second amplification

 P[4]

2T-1

CTA TTG TTA GAG GTT AGA GTC

nt 474–494

362

 P[6]

3T-1

TGT TGA TTA GTT GGA TTC AA

nt 259–278

146

 P[8]

1T-1

TCT ACT TGG ATA ACG TGC

nt 339 –356

224

 P[9]

4T-1

TGA GAC ATG CAA TTG GAC

nt 385–402

270

 P[10]

5T-1

ATC ATA GTT AGT AGT CGG

nt 575–594

462

  1. References: [35].