Figure 2

In vitro IL-8 production from AGS cells co-cultured with 6 ( dupA 1 ⁺ , cagA ⁺ , vacA ⁺ ) strains, 6 ( dupA 2 ⁺ , cagA ⁺ , vacA ⁺ ) and 6 ( dupA ⁻ , cagA ⁺ , vacA ⁺ ) strains. IL-8 production was significantly higher in dupA1⁺ (cagA ⁺, vacA ⁺) compared to dupA2⁺ (cagA ⁺, vacA ⁺) (536.0 ± 100.4 pg/mL, P = 0.008) and dupA-negative strains (cagA ⁺, vacA ⁺ ) (549.7 ± 104.1 pg/mL, P = 0.009). MOI of H. pylori strain was 100 for 8 hours. Experiments were repeated 3 times for the 18 strains. IL-8 in the supernatant was assayed by an ELISA in duplicate. Data are expressed as mean ± standard error.