Figure 3
Phylogenetic tree constructed based on 1.8 kb segment of dupA gene of H. pylori (determined in this study and reported by others). This Maximum Likelihood tree was generated using Tamura 3 parameter model in MEGA6 software (version 6.0.5, AZ, USA). Representative strains from India are marked in Red dots (●, red circle). Sequences of non Indian strains were used here from public database. Indian and East Asian strains formed one cluster called group I and Brazilian and Colombian strains formed another cluster called group II. The length of the vertical bar indicates the number of nucleotide substitution per site. Bootstrap values of ≥ 70 are indicated at the nodes. H. pylori strain designations indicate the geographic origins, as follows: F, Japan; WH, China; PG or I or SNT, India; HP, Brazil; C, Colombia. GenBank: accession no. for the strains used in the study are given in the parentheses: F228 [GenBank: AB617836.1], F77 [GenBank: AB617834.1], F58 [GenBank: AB617835.1], F64 [GenBank: AB617833.1], WH-34 [GenBank: KC707844.1], WH-33 [GenBank: KC707843.1], F51 [GenBank: AB617832.1], PG127 [GenBank: Submission in process], PG135-1a [GenBank: JN379048.1], PG186 [GenBank: KC894690.1], WH-2 [GenBank: KC707837.1], WH-29 [GenBank: KC707842.1], WH-15 ([GenBank: KC707839.1], I-87 [GenBank: KC894692.1], I-121 [GenBank: KC894689.1], SNT49 GenBank: CP002983.1], Hp166.03 [GenBank: HM770857.1], Hp896.95 [GenBank: HM770862.1], Hp178.02 [GenBank: HQ228198.1], C142 [GenBank: AB196363.1], Hp1335.95 [GenBank: HQ228195.1], Hp436.95 [GenBank: HQ228197.1].