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Table 1 List of primer used for Amplification and sequencing of dupA gene

From: Association of Intact dupA (dupA1) rather than dupA1 cluster with duodenal ulcer in Indian population

Primer Sequence (5′-3′) PCR cycling condition Amplicon size (bp)
SNT49F ATGTTTCTTGGTTTAGAGGG 94°C 30 sec, 55°C 30 sec and 72°C 2.5 mins 2499
dupA2499R TCACACATATTGAACATTCTCG
dupAF ATGAGTTCTGTATTAACAGACTTTG 94°C 30 sec, 50°C 30 sec and 72°C 2 mins 1884
dupA2499R TTAAATACTCTTCCTTATAAGTTTC
SNT49F ATGTTTCTTGGTTTAGAGGG 94°C 30 sec, 55°C 30 sec and 72°C 1 mins 685
SNT49R685 CAGCGTATAAATTCAATAGATC
dupAF ATGAGTTCTGTATTAACAGACTTTG 94°C 30 sec, 50°C 30 sec and 72°C 1.5 mins 1172
dupA20R CCTAAATTTTTGGCAATTTCTAATAAG
dupA16F ACAATACTGCTAATACAGATG 94°C 30 sec, 55°C 30 sec and 72°C 1 min 947
dupA2499R TCACACATATTGAACATTCTCG
SNT49_470F ATGATTTTAAATTATGTAGAGACC 94°C 30 sec, 55°C 30 sec and 72°C 40 secs 623
SNT49R350 GCATTAACAATTTTTTTAGCG
918 F CCTATATCGCTAACGCGCTC 94°C 30 sec, 55°C 30 sec and 72°C 40 secs 791
jhp0919R CTTTTTGTGATTTCATGAAACTC