First description of plasmid-mediated quinolone resistance determinants and β-lactamase encoding genes in non-typhoidal Salmonella isolated from humans, one companion animal and food in Romania

Table 2 Primers used for PCR

Primer	Sequence (5′–3′)	Target gene	Product (bp)	References
β-Lactamases
TEM-F	CATTTCCGTGTCGCCCTTAT	bla _TEM	793	[45]
TEM-R	TCCATAGTTGCCTGACTCCC	bla _TEM	793	[45]
PSE-1-F	AATGGCAATCAGCGCTTCCC	bla _PSE-1	586	[46]
PSE-1-R	GGGGCTTGATGCTCACTACA	bla _PSE-1	586	[46]
OXA-F	ACCAGATTCAACTTTCAA	bla _OXA	590	[47]
OXA-R	TCTTGGCTTTTATGCTTG	bla _OXA	590	[47]
SHV-F	TTATCTCCCTGTTAGCCACC	bla _SHV	795	[48]
SHV-R	GATTTGCTGATTTCGCTCGG	bla _SHV	795	[48]
CTX-M-F	ACCGCGATATCGTTGGT	bla _CTX-M	550	[49]
CTX-M-R	TTAGTGACCAGAATCAGCGG	bla _CTX-M	550	[49]
Plasmid-mediated quinolone resistance
qnrA-F	ATTTCTCACGCCAGGATTTG	qnrA	516	[35]
qnrA-R	GATCGGCAAAGGTTAGGTCA	qnrA	516	[35]
qnrB-F	GATCGTGAAAGCCAGAAAGG	qnrB	476	[35]
qnrB-R	ACGATGCCTGGTAGTTGTCC	qnrB	476	[35]
qnrS-F	ACGACATTCGTCAACTGCAA	qnrS	417	[35]
qnrS-R	TAAATTGGCACCCTGTAGGC	qnrS	417	[35]
aac(6′)-Ib-cr-F	CCCGCTTTCTCGTAGCA	aac(6′)-Ib-cr	544	[50]
aac(6′)-Ib-cr-R	TTAGGCATCACTGCGTCTTC	aac(6′)-Ib-cr	544	[50]
qepA-F	CGTGTTGCTGGAGTTCTTC	qepA	403	[51]
qepA-R	CTGCAGGTACTGCGTCATG	qepA	403	[51]

ISSN: 1757-4749