Cell cycle analysis of human epithelial Henle-407 cells infected with non-typhoidal Salmonella wild type (WT) strains and their corresponding isogenic cdtB mutants. Cells infected with Salmonella WT strains and their isogenic mutants were analyzed by FACS at 72 h of post-infection. Data shown represent the averages of two independent biological replicates with two technical replicates (two infected wells per experiment) each. Y axis indicates percentage of cells in G1, S, and G2/M phase (as indicated on the X axis); the bars indicate the range of values. Cells infected with Salmonella WT parent strains (i.e., S. Montevideo, S. Schwarzengrund, and S. Javiana) showed significantly higher percentage cells in G2/M phase compared to the corresponding isogenic ∆cdtB mutants (p < 0.0001 for the factor ∆cdtB deletion; ANOVA). Conversely, cells infected with the isogenic ∆cdtB mutants showed significantly higher percent cells in G1 phase (p < 0.0001; ANOVA) compared to the corresponding parent WT strains.