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Fig. 1 | Gut Pathogens

Fig. 1

From: The O-antigen negative ∆wbaV mutant of Salmonella enterica serovar Enteritidis shows adaptive resistance to antimicrobial peptides and elicits colitis in streptomycin pretreated mouse model

Fig. 1

Schematic presentation of final steps of dideoxy sugar biosynthesis, location of genes on chromosome and LPS profile of wild-type S. Enteritidis and its isogenic mutants. a CDP-paratose is synthesized by CDP-4-keto-3,6-dideoxy-d-glucose by the action of prt which is epimerized by Tyv to tyvelose. After synthesis, tyvelose is transferred to OAg backbone of mannose (Man), rhamnose (Rha) and galactose (Gal) by WbaV. CDP-abequose is also synthesized from same CDP sugar intermediate as CDP-paratose. b Location of genes on chromosome. The regions enclosed by the black bar were deleted with the help of lambda red recombinase system. White arrow indicate that genes are located on negative strand. c LPS from wild-type Salmonella as well as the mutants were isolated using the protocol described in materials and methods and separated on polyacrylamide gel electrophoresis (PAGE) gel using tricine-SDS buffer system. LPS were visualized by silver staining. c∆ indicates the mutants are complemented with the corresponding genes

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